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SIMPLE DESKTOPS OCTOPUS FULL

Next, we input the crude lysate directly into the iGenomX Riptide kit at 1/2x scale.

We then take a small aliquot of the cultures, gently lyse the cells to preferentially release plasmid over genomic DNA, and save the remainder as glycerol stocks.

Also, colony pickers typically cost >$100K and don’t seem appropriate for our scale. Because we usually need 400-1200 colonies, it’s still easier and faster to do this manually than by colony picker.

First, we pick colonies into normal 96-well plates and grow them overnight.

Here’s how OCTOPUS works in broad strokes:

SIMPLE DESKTOPS OCTOPUS FULL

We needed a robust, scalable, and low touch method for verifying full plasmid sequences - ideally one that would not require a huge investment in labor and capital. It became clear that our needs had outgrown the traditional approach. This is not ideal because it doesn’t find errors and mutations elsewhere in the plasmid, which when cloning at this scale, happen more than one would think. In addition, we had to limit ourselves to only sequencing the parts of the plasmid we were changing. For example, manually analyzing the data was about as painful as waiting for the results. There were other limits to the existing Sanger-based process as well. They could commit to 192 individual reads per business day, which meant if we wanted to sequence 1000 colonies with four reads each, we could be waiting weeks for results. Last year, as we scaled our cloning, we quickly outgrew our sequencing provider’s guaranteed turn-around time.

This new partnership will help IT and security managers in making their employees much happier and their domain dramatically more secure.At Octant, we use a pooled cloning strategy that can generate hundreds of unique clones in a single reaction. "By providing a seamlessly integrated desktop MFA, and universal passwordless authentication across the enterprise, HCM and Secret Double Octopus enable companies to make the most out of their ForgeRock deployments. "We are thrilled to partner with Hub City Media and help more ForgeRock users simplify security for their employees," said Raz Rafaeli, CEO and co-founder, Secret Double Octopus. With HCM and SDO as key partners for ForgeRock, it was a great opportunity to go to market together." "Secret Double Octopus has a similar focus, and has created a desktop authentication product that is the perfect fit for ForgeRock clients, and optimizes their platform. "We see a large amount of workforce IAM deployments, and this has become a focus for us over the years," said Phillippe Monrougie, CEO of Hub City Media. Passwordless Authentication enhances workforce security while providing a frictionless user experience. Its seamless integration with ForgeRock's identity platform offers a novel plug-and-play desktop MFA for the entire workforce, and a clear path to becoming a passwordless enterprise.Īs an expert in IAM deployments, HCM will work to seamlessly integrate ForgeRock and SDO with client environments to secure enterprise assets - applications, desktop, mobile and more. The Octopus platform is to date the only enterprise-grade solution able to solve any authentication use-case, from the workstation to any app and service, in a simple and secure manner.

SDO is revolutionizing workforce authentication with its Octopus Passwordless Enterprise™ technology, designed and built from the ground up for the unique requirements of complex enterprise infrastructure.

With over 20 years of IAM experience, HCM's extensive expertise in the industry continues to make them a leading partner for security platforms from leaders such as ForgeRock, Oracle and CyberArk. HCM offers advisory and implementation services alongside managed cloud and support services across the globe for a wide range of industries. Secret Double Octopus (SDO), the leader in enterprise passwordless authentication, and winner of ForgeRock's Global Partner Award for Workforce Technology, is partnering with Hub City Media (HCM), an Identity and Access Management (IAM) consultancy and ForgeRock's 2020 Americas Partner of the Year.